Flow cytometry is a technology used to measure complex cell phenotype and functions including:
- Cell surface markers
- Intracellular markers
- Cytokine secretion
- Phosphoprotein signalling
- Cell proliferation
- Cell viability and apoptosis
- DNA content
- Cell cycle.
The Telethon Kids Flow Facility is equipped with 3 flow cytometers/analysers, one cell sorter and one cell separator.
BD Symphony A5SE
- 355nm (UV) 405nm (Violet), 488nm (Blue), 561nm (Yellow/Green) and 640nm (Red) excitation lasers
- 48 fluorescence parameters
- Enables both spectral unmixing and compensation workflows
- High-Sensitivity GaAs PMTs Enables square form fit and gallium arsenide (GaAs) PMTs for enhanced resolution
- HTS sampling from 96 and 384 well plates
- Algorithmically Optimized Filter Set
- BD FACS Diva Software
BD LSR Fortessa
- 355nm (UV) 405nm (Violet), 488nm (Blue), 561nm (Yellow/Green) and 640nm (Red) excitation lasers
- 18 fluorescence parameters
- HTS sampling from 96 and 384 well plates
- BD FACS Diva Software
BD LSR Fortessa X-20
- 355nm (UV) 405nm (Violet), 488nm (Blue), 561nm (Yellow/Green) and 640nm (Red) excitation lasers
- 18 fluorescence parameters
- HTS sampling from 96 and 384 well plates
- BD FACS Diva Software
Flow Cytometer Sorters
BD FACSAria Fusion Cell Sorter
- 355nm (UV) 405nm (Violet), 488nm (Blue), 561nm (Yellow/Green) and 640nm (Red) excitation lasers
- 18 fluorescence parameters
- BD FACS Diva Software
- 4-way sorting with multiple adaptors/devices
- 70u, 85u, 100u Nozzle size option
BD FACS Melody Cell Sorter
- 405nm (Violet), 488nm (Blue), and 640nm (Red) excitation lasers
- 9 fluorescence parameters
- Fixed 100u nozzle size
- FACS Chorus Software
- 4-way sorting with multiple adaptors/devices
- Automated stream setup and steering into tubes or plates saves time