Successful establishment of primary small airway cell cultures in human lung transplantation

The study of small airway diseases such as post-transplant bronchiolitis obliterans syndrome (BOS) is hampered by the difficulty in assessing peripheral airway

Authors:
Banerjee B, Kicic A, Musk M, Sutanto EN, Stick SM, Chambers DC

Authors notes:
Respiratory Research 2009;10:99

Keywords:
primary small airway cell cultures, human lung transplantation

Abstract:
The study of small airway diseases such as post-transplant bronchiolitis obliterans syndrome (BOS) is hampered by the difficulty in assessing peripheral airway function either physiologically or directly. Our aims were to develop robust methods for sampling small airway epithelial cells (SAEC) and to establish submerged SAEC cultures for downstream experimentation.