Skip to content
The Kids Research Institute Australia logo
Donate

Discover . Prevent . Cure .

Interpretation of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine is adversely affected by methodological inaccuracies when using a commercial ELISA

The DNA lesion 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), a urinary marker of oxidative stress, is produced from reactions of reactive oxygen

Authors:
Garratt LW, Mistry V, Singh R, Sandhu JK, Sheil B, Cooke MS, Sly PD

Collaborators
Balding E, Berry LJ, Brennan S, Carlin JB, Carzino R, de Klerk N, Douglas T, Foo C, Gangell CL, Hall GL, Harrison J, Kicic A, Laing IA, Logie KM, Massie J, Mott LS, Murray C, Parsons F, Pillarisetti N, Poreddy SR, Ranganathan SC, Robertson CF, Robins-Browne R, Robinson PJ, Skoric B, Stick SM, Sutanto EN, Williamson E.

Authors notes:
Free Radical Biology and Medicine 2010;48:1460-64

Keywords:
urinary 8-oxo-7, 8-dihydro-2'-deoxyguanosine, adversely affected by methodological inaccuracies, using a commercial ELISA

Abstract:
The DNA lesion 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), a urinary marker of oxidative stress, is produced from reactions of reactive oxygen species with host DNA 2'-deoxyribonucleotides. The current gold-standard assessment is by complex chromatographic methods using HPLC or LC-MS/MS.