Authors:
Waithman J, Zanker D, Xiao K, Oveissi S, Wylie B, Ng R, et al.
Authors notes:
PLoS ONE. 2013;8(6):e66136
Keywords:
Dendritic cell subsets, CD8+ T lymphocyte repines, MHC class II, influenza, infection
Abstract:
The identification of the specific DC subsets providing a critical role in presenting influenza antigens to naïve T cell precursors remains contentious and under considerable debate.
Here we show that CD8+ T lymphocyte (TCD8+) responses are severely hampered in C57BL/6 mice deficient in the transcription factor Batf3 after intranasal challenge with influenza A virus (IAV).
This transcription factor is required for the development of lymph node resident CD8+ and migratory CD103+CD11b- DCs and we found both of these subtypes could efficiently stimulate anti-IAV TCD8+.
Using a similar ex vivo approach, many publications on this subject matter excluded a role for resident, non-migratory CD8+ DC.
We postulate the differences reported can partially be explained by how DC are phenotyped, namely the use of MHC class II to segregate subtypes.
Our results show that resident CD8+ DC upregulate this marker during IAV infection and we advise against its use when isolating DC subtypes.