The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication

We report a role for the mitochondrial single-stranded DNA binding protein (mtSSB) in regulating mitochondrial DNA (mtDNA) replication initiation in mammalian mitochondria. Transcription from the light-strand promoter (LSP) is required both for gene expression and for generating the RNA primers needed for initiation of mtDNA synthesis.

Citation:
Jiang M, Xie X, Zhu X, Jiang S, ....... Sirra SJ, Carelli V, Griffith JD, Filipovska A, et al. The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication. Sci Adv. 2021;7(27).

Keywords:
Mitochondiral; DNA binding protein

Abstract:
We report a role for the mitochondrial single-stranded DNA binding protein (mtSSB) in regulating mitochondrial DNA (mtDNA) replication initiation in mammalian mitochondria. Transcription from the light-strand promoter (LSP) is required both for gene expression and for generating the RNA primers needed for initiation of mtDNA synthesis.