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In Vitro primary human airway epithelial whole exhaust exposure

The method outlined in this article is a customization of the whole exhaust exposure method generated by Mullins et al. (2016) using reprogrammed primary human airway epithelial cells as described by Martinovich et al. (2017). It has been used successfully to generate recently published data (Landwehr et al. 2021). The goal was to generate an exhaust exposure model where exhaust is collected from a modern engine, real-world exhaust concentrations are used and relevant tissues exposed to assess the effects of multiple biodiesel exposures.

Citation:
Landwehr KR, Hillas J, Mead-Hunter R, Brooks P, King A, …… Kicic A, Mullins BJ, Larcombe AN. In Vitro primary human airway epithelial whole exhaust exposure. MethodsX. 2021;8:101561.

Keywords:
Airway epithelial cells; Biodiesel; Environmental exposure; Exhaust exposure; Exposure protocol

Abstract:
The method outlined in this article is a customization of the whole exhaust exposure method generated by Mullins et al. (2016) using reprogrammed primary human airway epithelial cells as described by Martinovich et al. (2017). It has been used successfully to generate recently published data (Landwehr et al. 2021). The goal was to generate an exhaust exposure model where exhaust is collected from a modern engine, real-world exhaust concentrations are used and relevant tissues exposed to assess the effects of multiple biodiesel exposures.