Digital RNase Footprinting of RNA-Protein Complexes and Ribosomes in Mitochondria

RNA-binding proteins and mitochondrial ribosomes have been found to be linchpins of mitochondrial gene expression in health and disease. The expanding repertoire of proteins that bind and regulate the mitochondrial transcriptome has necessitated the development of new tools and methods to examine their molecular functions.

Citation:
Rudler DL, Siira SJ, Rackham O, Filipovska A. Digital RNase Footprinting of RNA-Protein Complexes and Ribosomes in Mitochondria. Methods in Molecular Biology. 2661: Humana Press Inc.; 2023. p. 317-28.

Keywords:
Bioinformatics; Footprinting; Mitoribosome; RNA-Seq; RNA-binding proteins; mtDNA

Abstract:
RNA-binding proteins and mitochondrial ribosomes have been found to be linchpins of mitochondrial gene expression in health and disease. The expanding repertoire of proteins that bind and regulate the mitochondrial transcriptome has necessitated the development of new tools and methods to examine their molecular functions.