Single-nucleus RNA sequencing of pre-malignant liver reveals disease-associated hepatocyte state with HCC prognostic potential

Current approaches to staging chronic liver diseases have limited utility for predicting liver cancer risk. Here, we employed single-nucleus RNA sequencing (snRNA-seq) to characterize the cellular microenvironment of healthy and pre-malignant livers using two distinct mouse models.

Citation:
Carlessi R, Denisenko E, Boslem E, ……… Tjiam MC, Lister R, Karin M, et al. Single-nucleus RNA sequencing of pre-malignant liver reveals disease-associated hepatocyte state with HCC prognostic potential. Cell Genom; 2023 3(5) 100301

Keywords:
Cancer surveillance; chronic liver disease; daHep; disease-associated hepatocyte; HCC; hepatocellular carcinoma; pre-malignancy; prognostic biomarker; snRNA-seq

Abstract:
Current approaches to staging chronic liver diseases have limited utility for predicting liver cancer risk. Here, we employed single-nucleus RNA sequencing (snRNA-seq) to characterize the cellular microenvironment of healthy and pre-malignant livers using two distinct mouse models.