Use of high-resolution fluorescence in situ hybridization for fast and robust detection of SARS-CoV-2 RNAs

Early, rapid, and accurate diagnostic tests play critical roles not only in the identification/management of individuals infected by SARS-CoV-2, but also in fast and effective public health surveillance, containment, and response. Our aim has been to develop a fast and robust fluorescence in situ hybridization (FISH) detection method for detecting SARS-CoV-2 RNAs by using an HEK 293 T cell culture model.

Citation:
Hu J, Hu J, Jin L, ……. Wang T, Ren Y, et al. Use of high-resolution fluorescence in situ hybridization for fast and robust detection of SARS-CoV-2 RNAs. Sci Rep. 2024;14(1).

Keywords:
COVID-19; Fluorescence in situ hybridization; Infection; RNA virus; SARS-CoV-2; mRNA.

Abstract:
Early, rapid, and accurate diagnostic tests play critical roles not only in the identification/management of individuals infected by SARS-CoV-2, but also in fast and effective public health surveillance, containment, and response. Our aim has been to develop a fast and robust fluorescence in situ hybridization (FISH) detection method for detecting SARS-CoV-2 RNAs by using an HEK 293 T cell culture model.