Investigators: Asha Bowen
External collaborators:
Bart Currie (Menzies School of Health Research)
Josh Francis (Menzies School of Health Research)
Deborah Holt (Menzies School of Health Research)
James McCarthy (The Council of the Queensland Institute of Medical Research)
Cielo Pasay (QIMR Berghofer Medical Research Institute)
Matt Field (James Cook University)
Tony Papenfuss (The Walter and Eliza Hall Institute of Medical Research)
Katja Fisher (QIMR Berghofer Medical Research Institute)
Kate Mounsey (University of Sunshine Coast)
Dev Tilakaratne (Royal Darwin Hospital)
"In recent years, the interest in molecular diagnostic methods for the detection of many pathogens has grown substantially. This escalation in interest has occurred in parallel with data indicating inaccuracy of scabies diagnosis based on currently available methods such as handheld dermatoscopy, burrow ink test and examination of skin samples by standard microscopy. The paucity of mites in classical scabies makes it extremely difficult for even an experienced dermatologist to make a definitive diagnosis. Hence, scabies can be easily misdiagnosed as an allergic reaction or eczema.
We propose to develop a real-time PCR assay for the detection of human scabies. Using this tool, we will search the genome of scabies for highly repetitive, non-coding DNA elements in order to identify diagnostic targets capable of providing a more sensitive and species-specific detection. Using these more abundant, high copy number repeats as targets, we will design a probe-based real time PCR that can detect low level infections in scabies-infected individuals. Through improved diagnostic sensitivity, this platform should facilitate improved diagnosis of scabies.